|Krisnanda DA||Yogyakarta State Universityemail@example.com|
|THE EFFECT OF HONEY SUPPLEMENTATION BEFORE PHYSICAL ACTIVITY TOWARDS THE PLASMA MALONDIALDEHYDE LEVEL IN MALE WISTAR RATS (RATTUS NORVEGICUS)|
|honey, physical activity, plasma MDA, Wistar rats, Health|
Objectives: An exercise has positive and negative impacts. The benefits of exercise are improving the fitness and health of a body, improving performance, and preventing several diseases. One of the negative effects of exercise is the formation of oxidant compounds. The oxidant compounds can trigger oxidative stress, a condition caused by the imbalance between the production of free radicals and the antioxidants. Malondialdehyde (MDA) is one of the biological markers of oxidative stress in organism. Oxidative stress can be prevented by giving exogenous antioxidants. Honey is a rich source of antioxidants. The objective of this study aims to determine the effect of honey supplementation before physical activity towards reduction levels on plasma malondialdehyde (MDA) in male rats. Methods:The study was an experimental laboratory with the posttest only control group design and which was conducted in 7 days. The samples of this study were using 20 male white strain Wistar rats (Rattus norvegicus). The rats were divided into 2 groups randomly and each group consists of 10 rats. The two groups are: K0 = control group (mean of weight 170,89±12,12, physical activity 148,44±11,28 and MDA plasma 12.02±1,85), K1 = supplemented honey 5 g / kg body weight (mean of weight 170,89±12,12, physical activity 154,22±11,60 and MDA plasma 7,75±1,61). Swimming 70% of maximum capacity was used as physical activity with the additional weights of 6% from rat body weight. Honey supplementation given once daily for 7 days by oral feeding via sonde. The data were analyzed using statistical program SPSS 16. Results:The results showed that the mean levels of plasma MDA in group K0 was 12.02±1.85 nmol/ml and 7.75±1.61 nmol/ml in group K1. Independent sample t-test showed a significant difference p = 0.000 (p <0.05). Reduction levels of plasma MDA was 35.52%. Conclusions: Independent sample t test showed a significant differences among the groups (p<0.05). Supplemented honey with 5 g / kg gave the significant decrease in MDA levels. The results can be used as a basic knowledge for further research to determine its use in human being.